Discovery Biology

 

Ethix Pharma offer a comprehensive library of cell biology based methods & models to assess the molecular action of the test formulations. We can customize a range of in vitroex vivo and in vivo cell based models to determine the key cellular pathways determining the pharmacology of the lead molecules. One or more of the following strategies may be undertaken to profile the molecule under investigation

 

 

Screening methods available with us are:

Therapeutic Area Modules

Cancer

  • Cytotoxicity assay by MTT/SRB/Trypan blue
  • Clonogenic assay
  •     Apoptosis - Nuclear staining with DAPI
     Alkylating activity assay
      TUNEL assay
  • Endonuclease assay
  • DNA fragmentation analysis / DNA ladder assay
  • Cancer specific gene expression analysis
  • (P53, BCl2, Caspase-3, VEGF, cyclin D1, cyclin B1, STAT3 etc.)
  • Hepatoprotective
  • Hepatoprotective study against selected toxicant.
  • Hepatoprotective study in isolated primary rat hepatocytes.
  • Gene expression studies
  • Diabetes
  • Glucose uptake assay in skeletal muscle cells (Non-radio labeled assay).
  • Glucose uptake in isolated rat hemi diaphragm
  • Gene expression studies (PPARγ, GLUT-4 etc)
  • Alpha-Amylase and Alpha-galactosidase inhibitory activity
  • Alpha-glucosidase activity
  • Immunomodulatory and Inflammation
  • Phagocytic assay on cellular lysosomal enzyme activity
  • Proliferation assay
  • NO and TNF inhibitory studies
  • HRBC membrane stabilization
  • Inhibition of protein denaturation
  • Proteinase inhibitory action
  • Analysis of markers like COX-1, COX-2, PGE-2, iNOS, IFN-alpha, IFN-gamma, TNF-alpha, Interleukins 1, 10, 12, 18 etc.
  • Antioxidant
  • DPPH scavenging assay
  • Scavenging of Nitric Oxide radical
  • Lipid peroxidation inhibitory activity
  • Evaluation of Total Antioxidant capacity
  • Ferric ion reducing power assay
  • Scavenging of Hydroxyl Radical by p-NDA Method
  • Scavenging of Hydrogen Peroxide
  • Scavenging of ABTS radical cation Assay
  • Total Phenolic and Flavonoid content estimation
  • Obesity
  • Anti adepogensis studies in 3T3L-1 pre adepocytes by
  • Oil Red-O staining
  • Cell triglyceride assay
  • Gene expression studies (PPAR-gamma, SREBF etc)
  • Anti infective
  • Anti microbial activity by Zone of inhibition.
  • Anti microbial activity by MIC determination
  • Antibiotic sensitivity testing
  • TLC bioautography
  • In vitro anti viral studies
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      Discovery Biology
      Toxicology
      Pharmacology

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